Neuroscience
Bertaina-Anglade V, Enjuanes E, Morillon D, & Drieu la Rochelle C. (2006). The object recognition task in rats and mice: A simple and rapid model in safety pharmacology to detect amnesic properties of a new chemical entity. J Pharmacol Toxicol Methods, 54(2), 99-105.
Preclinical Pharmacology Department, Biotrial, 7-9 rue JL Bertrand, 35000 Rennes, France.
INTRODUCTION: The aim of the present study was to evaluate the potential usefulness of the object recognition learning paradigm to detect the potential amnesic properties of a new drug for use in the characterisation of its safety pharmacology profile. METHODS AND RESULTS: In the first experiment, the time-dependent decay of object recognition memory was characterised in Sprague-Dawley rats and C57Bl/6J mice. Under our experimental conditions, it takes between 3 and 4 post-training hours for the rats and between 1 and 2 post-training hours for the mice to forget the respective value of the objects. In the second experiment, the effects of scopolamine (0.03-1 mg/kg) were investigated in both rats and mice when administered 30 min prior to training in the object recognition task. Memory retention was tested 2 h after training in rats and 1 h after training in mice. Scopolamine impairs the object recognition memory at doses of 0.1, 0.3, and 1 mg/kg in rats and at doses of 0.3 and 1 mg/kg in mice. In the last experiment, effects of two benzodiazepines (alprazolam and diazepam) were assessed in the mouse model of object recognition task. Diazepam and alprazolam were intraperitoneally administered 30 min prior to training and memory retention was tested 10 min and 1 h after training. At 0.2 mg/kg, both benzodiazepines impair object recognition memory when testing is performed 1 h after training. However, when testing is performed 10 min after training, both benzodiazepines at 0.2 mg/kg failed to disrupt memory processes. DISCUSSION: Taken together, these results show that the object recognition task can easily be performed in rats and mice for safety pharmacology studies related to CNS function. Because of the ageing population and the increasing number of drugs prescribed to elderly patients, it becomes important to evaluate the potential side effects of a new chemical entity on memory function during evaluation of its safety profile. The object recognition task, which is simple, rapid, and reliable, should be of great use in safety pharmacology to detect amnesic properties of new compounds.
PMID: 16750402 [PubMed - indexed for MEDLINE]
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Anthony H. Risser | neuroscience | neuropsychology | brain
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Haramati S, Soroker N, Dudai Y, & Levy DA. The posterior parietal cortex in recognition memory: A neuropsychological study. Neuropsychologia. 2007 Nov 29 [Epub ahead of print] Department of Neurobiology, Weizmann Institute of Science, P.O. Box 26, Rehovot...
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- Abstract Of The Day: Memory Distortion
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Neuroscience
Abstract of the Day: Assessing Neurocognitive Toxicity in Animal Models
Bertaina-Anglade V, Enjuanes E, Morillon D, & Drieu la Rochelle C. (2006). The object recognition task in rats and mice: A simple and rapid model in safety pharmacology to detect amnesic properties of a new chemical entity. J Pharmacol Toxicol Methods, 54(2), 99-105.
Preclinical Pharmacology Department, Biotrial, 7-9 rue JL Bertrand, 35000 Rennes, France.
INTRODUCTION: The aim of the present study was to evaluate the potential usefulness of the object recognition learning paradigm to detect the potential amnesic properties of a new drug for use in the characterisation of its safety pharmacology profile. METHODS AND RESULTS: In the first experiment, the time-dependent decay of object recognition memory was characterised in Sprague-Dawley rats and C57Bl/6J mice. Under our experimental conditions, it takes between 3 and 4 post-training hours for the rats and between 1 and 2 post-training hours for the mice to forget the respective value of the objects. In the second experiment, the effects of scopolamine (0.03-1 mg/kg) were investigated in both rats and mice when administered 30 min prior to training in the object recognition task. Memory retention was tested 2 h after training in rats and 1 h after training in mice. Scopolamine impairs the object recognition memory at doses of 0.1, 0.3, and 1 mg/kg in rats and at doses of 0.3 and 1 mg/kg in mice. In the last experiment, effects of two benzodiazepines (alprazolam and diazepam) were assessed in the mouse model of object recognition task. Diazepam and alprazolam were intraperitoneally administered 30 min prior to training and memory retention was tested 10 min and 1 h after training. At 0.2 mg/kg, both benzodiazepines impair object recognition memory when testing is performed 1 h after training. However, when testing is performed 10 min after training, both benzodiazepines at 0.2 mg/kg failed to disrupt memory processes. DISCUSSION: Taken together, these results show that the object recognition task can easily be performed in rats and mice for safety pharmacology studies related to CNS function. Because of the ageing population and the increasing number of drugs prescribed to elderly patients, it becomes important to evaluate the potential side effects of a new chemical entity on memory function during evaluation of its safety profile. The object recognition task, which is simple, rapid, and reliable, should be of great use in safety pharmacology to detect amnesic properties of new compounds.
PMID: 16750402 [PubMed - indexed for MEDLINE]
-
Anthony H. Risser | neuroscience | neuropsychology | brain
- Seeing What Things Are
As soon as we know something is there, we already know what category of thing it is. That's according to Kalanit Grill-Spector and Nancy Kanwisher at Stanford University and MIT. They showed people images for between 17 to 167ms, some of which contained...
- Neuropsychology Abstract Of The Day: Visuocognition
The Cambridge Car Memory Test: A task matched in format to the Cambridge Face Memory Test, with norms, reliability, sex differences, dissociations from face memory, and expertise effects Behav Res Methods. 2011 Oct 20; Dennett HW, McKone E, Tavashmi R,...
- Neuropsychology Abstract Of The Day: Parietal Function
Haramati S, Soroker N, Dudai Y, & Levy DA. The posterior parietal cortex in recognition memory: A neuropsychological study. Neuropsychologia. 2007 Nov 29 [Epub ahead of print] Department of Neurobiology, Weizmann Institute of Science, P.O. Box 26, Rehovot...
- Abstract Of The Day: Malnutrition
Nakagawasai O, Yamadera F, Sato S, Taniguchi R, Hiraga H, Arai Y, Murakami H, Mawatari K, Niijima F, Tan-No K, & Tadano T. Alterations in cognitive function in prepubertal mice with protein malnutrition: relationship to changes in choline acetyltransferase....
- Abstract Of The Day: Memory Distortion
Schacter DL, Slotnick SD. The cognitive neuroscience of memory distortion. Neuron. 2004 Sep 30;44(1):149-60. Department of Psychology, Harvard University, Cambridge, MA 02138 USA. Memory distortion occurs in the laboratory and in everyday life....